Difference between revisions of "Part:BBa K4165142"

 
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Tau binding peptide targeting the PHF seed of Tau
 
Tau binding peptide targeting the PHF seed of Tau
  
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===Usage and Biology===
 
===Usage and Biology===
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MMD3rev peptide sequence is YWVSTHRAKLPD, it binds to 275-VQIINK-280 (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* and full‐length Tau aggregates, but not PHF driven aggregates, and prevents the formation of tau β‐sheet‐rich fibrils.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4165142 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4165142 SequenceAndFeatures</partinfo>
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===Dry Lab===
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<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
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MMD3rev is modeled by AlphaFold2, Apptest, ITASSER and TrRosetta, best model obtained from ITASSER.
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmd3rev.png" style="margin-left:200px;" alt="" width="300" /></p>
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</html>
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                            Figure 1.: Predicted 3D structure of Synthetic peptide MMD3rev.
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<p style=" font-weight: bold; font-size:13px;"> Table 1: Quality assessment parameters of MMD3rev model. </p>
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmd3rev-qa.png" style="margin-left:75px;" alt="" width="800" /></p>
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</html>
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===References===
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1. Reference
  
  

Revision as of 19:44, 5 October 2022


MMD3rev Peptide

Tau binding peptide targeting the PHF seed of Tau

Usage and Biology

MMD3rev peptide sequence is YWVSTHRAKLPD, it binds to 275-VQIINK-280 (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* and full‐length Tau aggregates, but not PHF driven aggregates, and prevents the formation of tau β‐sheet‐rich fibrils.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Dry Lab

Modeling

MMD3rev is modeled by AlphaFold2, Apptest, ITASSER and TrRosetta, best model obtained from ITASSER. 


                            Figure 1.: Predicted 3D structure of Synthetic peptide MMD3rev.


Table 1: Quality assessment parameters of MMD3rev model.


References

1. Reference