Difference between revisions of "Part:BBa K4387986"
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<partinfo>BBa_K4387986 short</partinfo> | <partinfo>BBa_K4387986 short</partinfo> | ||
| − | This composite part contains an anti-TNF nanobody construct with a myc-tag and the HlyA-tag required for the secretion with the hemolysin A secretion system | + | This composite part contains an anti-TNF nanobody construct with a myc-tag and the HlyA-tag required for the secretion with the hemolysin A secretion system <partinfo>BBa_K4387979</partinfo>. The expression of the nanobody is under control of the inducible pBad system, followed by the T7 RBS. The myc-tag was required for the characterization of the secretion and functionality of the nanobody. The nanobody inserted in this part is the monovalent nanobody VHH#2B. |
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Revision as of 17:50, 5 October 2022
Monovalent nanobody expression with the HlyA secretion signal (VHH#2B)
This composite part contains an anti-TNF nanobody construct with a myc-tag and the HlyA-tag required for the secretion with the hemolysin A secretion system BBa_K4387979. The expression of the nanobody is under control of the inducible pBad system, followed by the T7 RBS. The myc-tag was required for the characterization of the secretion and functionality of the nanobody. The nanobody inserted in this part is the monovalent nanobody VHH#2B.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1650
Illegal BglII site found at 2229
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000COMPATIBLE WITH RFC[1000]