Difference between revisions of "Part:BBa K4179013:Design"

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===Design Notes===
 
===Design Notes===
 
The linker [https://parts.igem.org/Part:BBa_K4179004 (BBa_K4179004)] contains a 6xHis tag and a thrombin recognition and cleavage site.
 
The linker [https://parts.igem.org/Part:BBa_K4179004 (BBa_K4179004)] contains a 6xHis tag and a thrombin recognition and cleavage site.
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===References===
 

Latest revision as of 17:18, 5 October 2022


ChimeraI under Rhlr promoter + P2A + mCherry reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2152
    Illegal PstI site found at 547
    Illegal PstI site found at 1792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1357
    Illegal PstI site found at 547
    Illegal PstI site found at 1792
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2152
    Illegal PstI site found at 547
    Illegal PstI site found at 1792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2152
    Illegal PstI site found at 547
    Illegal PstI site found at 1792
    Illegal NgoMIV site found at 962
    Illegal AgeI site found at 1431
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker (BBa_K4179004) contains a 6xHis tag and a thrombin recognition and cleavage site.