Difference between revisions of "Part:BBa K190019:Design"

Revision as of 20:01, 10 September 2009

fMT

fMT is a metallothionein, binding Arsenite(III) and Arsenate(V), it has higher affinity for As(III).

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 38
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 127
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Add BioBrick prefix and suffix, also a RBS (B0034) will be added (not in sequence yet)

Source

fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of Fucus vesiculosus.

[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&RID=AJPJ1GVY016&log%24=nuclalign&blast_rank=1&list_uids=3819155 GenBank: AJ005834.1]

References

Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924–2927

@@ Line 13: / Line 13: @@
 ===Source===
-fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of ''Fucus vesiculosus''.
+fMT was cloned from pMAL-MT (kindly provided by Wilfred Chen, University of California), the origin of the gene is the genome of ''Fucus vesiculosus''.<BR><BR>
+[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&RID=AJPJ1GVY016&log%24=nuclalign&blast_rank=1&list_uids=3819155 GenBank: AJ005834.1]
 ===References===
 Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein. Singh et al. App and Env Microbiology, May 2008, p. 2924–2927