Difference between revisions of "Part:BBa J37032:Experience"

Revision as of 11:58, 10 September 2009

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Applications of BBa_J37032

User Reviews

UNIQe435879bfbbc0f2c-partinfo-00000000-QINU

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Aberdeen_Scotland 2009

Experimental Design
The J37032 BioBrick expresses GFP under control of a LuxR responsive promoter. The J37032 part was provided by iGEM-HQ, was succesfully mini-preppred and transformed into E.coli. J37032 was used to test our constitutive LuxR/LuxI operon (No part name specified with partinfo tag.

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 <b>Experimental Design</b><br>
-The <partinfo>BBa_J37032<\partinfo> BioBrick expresses GFP under control of a LuxR responsive promoter. The J37032 part was provided by iGEM-HQ, was succesfully mini-preppred and transformed into E.coli.  J37032 was used to test our constitutive LuxR/LuxI operon (<partinfo>BBa_K182200<\partinfo>). For that purpose SCS1 E.coli was transformed either with K182200 alone (referred to as pIR in figure below),  J37032 alone, or a combination of both plasmids. The single transformants acted as negative controls.
+The J37032 BioBrick expresses GFP under control of a LuxR responsive promoter. The J37032 part was provided by iGEM-HQ, was succesfully mini-preppred and transformed into E.coli.  J37032 was used to test our constitutive LuxR/LuxI operon (<partinfo>BBa_K182200<\partinfo>). For that purpose SCS1 E.coli was transformed either with K182200 alone (referred to as pIR in figure below),  J37032 alone, or a combination of both plasmids. The single transformants acted as negative controls.
 <b>Results</b><br>