Difference between revisions of "Part:BBa K4179010:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The linker [https://parts.igem.org/Part:BBa_K4179004 (BBa_K4179004)] contains a 6xHis tag and a thrombin recognition and cleavage site. | |
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Latest revision as of 16:33, 5 October 2022
UDT under Rhlr promoter + P2A + mCherry reporter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2134
Illegal PstI site found at 1774 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1339
Illegal PstI site found at 1774 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2134
Illegal PstI site found at 1774 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2134
Illegal PstI site found at 1774
Illegal AgeI site found at 1413 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The linker (BBa_K4179004) contains a 6xHis tag and a thrombin recognition and cleavage site.