Difference between revisions of "Part:BBa K4162005"
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| − | Ribozymes are non-coding RNAs that can catalyze specific biochemical reactions without the assistance of proteins, involved in a variety of vital cellular reactions. Ribozymes can be divided into RNA metalloenzymes and self-cleaving ribozymes. Studies have shown that self-cleaving ribozymes adopt a general acid-base catalytic mechanism to perform site-specific phosphodiester bond cleavage. Until now, only ten families of self-cleaving ribozymes have been discovered, and hammerhead ribozyme is one of them. By inserting the DNA sequence of hammerhead ribozyme between two coding sequences, mRNA transcribed by this gene will perform self cleavage at the ribozyme site, so that a long piece of mRNA will be cut into segments to prevent the translation process of various coding sequences from interfering with each other. | + | Ribozymes are non-coding RNAs that can catalyze specific biochemical reactions without the assistance of proteins, involved in a variety of vital cellular reactions. Ribozymes can be divided into RNA metalloenzymes and self-cleaving ribozymes. Studies have shown that self-cleaving ribozymes adopt a general acid-base catalytic mechanism to perform site-specific phosphodiester bond cleavage. Until now, only ten families of self-cleaving ribozymes have been discovered, and hammerhead ribozyme is one of them. By inserting the DNA sequence of hammerhead ribozyme between two coding sequences, mRNA transcribed by this gene will perform self cleavage at the ribozyme site, so that a long piece of mRNA will be cut into segments to prevent the translation process of various coding sequences from interfering with each other.<ref>Blount, K F, and O C Uhlenbeck. “The hammerhead ribozyme.” Biochemical Society transactions vol. 30,Pt 6 (2002): 1119-22. doi:10.1042/bst0301119</ref> |
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Revision as of 14:32, 5 October 2022
Hammerhead ribozyme
Ribozymes are non-coding RNAs that can catalyze specific biochemical reactions without the assistance of proteins, involved in a variety of vital cellular reactions. Ribozymes can be divided into RNA metalloenzymes and self-cleaving ribozymes. Studies have shown that self-cleaving ribozymes adopt a general acid-base catalytic mechanism to perform site-specific phosphodiester bond cleavage. Until now, only ten families of self-cleaving ribozymes have been discovered, and hammerhead ribozyme is one of them. By inserting the DNA sequence of hammerhead ribozyme between two coding sequences, mRNA transcribed by this gene will perform self cleavage at the ribozyme site, so that a long piece of mRNA will be cut into segments to prevent the translation process of various coding sequences from interfering with each other.[1]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Blount, K F, and O C Uhlenbeck. “The hammerhead ribozyme.” Biochemical Society transactions vol. 30,Pt 6 (2002): 1119-22. doi:10.1042/bst0301119