Difference between revisions of "Part:BBa K4192011"
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It encoding global regulator GacA. It can enhance extracellular polysaccharide (EPS) synthesis, enhance antifungal ability and weaken exercise ability<sup>[1]</sup>.
 
It encoding global regulator GacA. It can enhance extracellular polysaccharide (EPS) synthesis, enhance antifungal ability and weaken exercise ability<sup>[1]</sup>.
 
Therefore, when it is co-expressed with other regulatory genes, the production of biofilm can be significantly increased.
 
Therefore, when it is co-expressed with other regulatory genes, the production of biofilm can be significantly increased.
−
The two-component regulatory system, typically composed of a membrane-bound sensor kinase (GacS) and a cytoplasmic response protein (GacA), distribute widely in bacteria. A number of publications demonstrated that the GacS/GacA system globally regulated the production of the extracellular compounds and exoenzymes and played an important role in the biocontrol activity of the Pseudomonas spp<sup>[2]</sup>. GacA can regulate a variety of downstream physiological activities, so the effect is not single. It even increase the content of c-di-GMP, similar to the cdg gene (BBa_K4192010).
+
It belongs to a two-component regulatory system, typically composed of a membrane-bound sensor kinase (GacS) and a cytoplasmic response protein (GacA), distribute widely in bacteria. A number of publications demonstrated that the GacS/GacA system globally regulated the production of the extracellular compounds and exoenzymes and played an important role in the biocontrol activity of the Pseudomonas spp<sup>[2]</sup>. GacA can regulate a variety of downstream physiological activities, so the effect is not single. It even increase the content of c-di-GMP, similar to the cdg gene (BBa_K4192010).
  
 
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Revision as of 11:38, 5 October 2022


gacA from Pseudomonas fluorescens str. 2P24

It encoding global regulator GacA. It can enhance extracellular polysaccharide (EPS) synthesis, enhance antifungal ability and weaken exercise ability[1]. Therefore, when it is co-expressed with other regulatory genes, the production of biofilm can be significantly increased. It belongs to a two-component regulatory system, typically composed of a membrane-bound sensor kinase (GacS) and a cytoplasmic response protein (GacA), distribute widely in bacteria. A number of publications demonstrated that the GacS/GacA system globally regulated the production of the extracellular compounds and exoenzymes and played an important role in the biocontrol activity of the Pseudomonas spp[2]. GacA can regulate a variety of downstream physiological activities, so the effect is not single. It even increase the content of c-di-GMP, similar to the cdg gene (BBa_K4192010).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]



References

[1]Seaton, Sarah C et al. “Pleiotropic effects of GacA on Pseudomonas fluorescens Pf0-1 in vitro and in soil.” Applied and environmental microbiology vol. 79,17 (2013): 5405-10. doi:10.1128/AEM.00819-13

[2]Yan Xiaoxue.The role of regulatory gene gacA in the biocontrol activity of Pseudomonas fluorescens 2P24.2004.Northwest Sci-Tech University of Agriculture and Forestry,PhD dissertation..