Difference between revisions of "Part:BBa K4345018"

 
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Design of the thermoregulated translation of toxin ccdB. Fluorescent protein sfGFP was linked to the antitoxin for visualisation.
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<!-- Add more about the biology of this part here

Revision as of 10:56, 5 October 2022


Tet/lac promoter - ccdB fused to sfGFP

Design of the thermoregulated translation of toxin ccdB. Fluorescent protein sfGFP was linked to the antitoxin for visualisation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 352
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]