Difference between revisions of "Part:BBa K4137001"
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<partinfo>BBa_K4137001 short</partinfo>
 
<partinfo>BBa_K4137001 short</partinfo>
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BBa_K4137001 codes for the CcdB protein, a toxin to many bacteria including E. coli and B. subtilis. Through the formation of  
 
BBa_K4137001 codes for the CcdB protein, a toxin to many bacteria including E. coli and B. subtilis. Through the formation of  
 
a CcdA-CcdB complex by binding with CcdA, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. A Myc tag is added for protein purification, identification, and distinguishment from CcdA.
 
a CcdA-CcdB complex by binding with CcdA, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. A Myc tag is added for protein purification, identification, and distinguishment from CcdA.

Revision as of 06:47, 5 October 2022


CcdB-Myc Toxin

BBa_K4137001 codes for the CcdB protein, a toxin to many bacteria including E. coli and B. subtilis. Through the formation of a CcdA-CcdB complex by binding with CcdA, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. A Myc tag is added for protein purification, identification, and distinguishment from CcdA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216