Difference between revisions of "Part:BBa K4244004"
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The start codon of sfGFP (<partinfo>BBa_K3143689</partinfo>) was removed to allow N-terminus fusion with signal peptide | The start codon of sfGFP (<partinfo>BBa_K3143689</partinfo>) was removed to allow N-terminus fusion with signal peptide | ||
| − | A silent mutation was also performed to removed | + | A silent mutation was also performed to removed the SapI site present in <partinfo>BBa_K3143689</partinfo> at poisition 13 (A to G) to make it compatible with RFC[1000] |
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Revision as of 06:44, 5 October 2022
sfGFP without start codon
The start codon of sfGFP (BBa_K3143689) was removed to allow N-terminus fusion with signal peptide A silent mutation was also performed to removed the SapI site present in BBa_K3143689 at poisition 13 (A to G) to make it compatible with RFC[1000]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]