Difference between revisions of "Part:BBa K4137000:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
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The Myc purification tag is added to the N-terminal of the protein; structural analysis was conducted to verify that the tag did not excessively interfere with protein structure.
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The 6xHis purification tag was added to the C-terminal of the protein. The sequence has been codon optimized for E. coli.
  
 
===Source===
 
===Source===

Revision as of 06:28, 5 October 2022


CcdA-6xHis Antitoxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 6xHis purification tag was added to the C-terminal of the protein. The sequence has been codon optimized for E. coli.

Source

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1

References