Difference between revisions of "Part:BBa K4204002"
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| − | + | In this sequence, we first integrated protection bases at the 5’ end of the sequence, this is to protect the sequence from damage. And immediately following the T7 promoter， connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-16s region pairs with the targeted B.cereus 16srRNA sequence and enables cleavage. | |
Revision as of 02:16, 5 October 2022
In this sequence, we first integrated protection bases at the 5’ end of the sequence, this is to protect the sequence from damage. And immediately following the T7 promoter， connecting with the sgRNA scaffold, which helps the folding of sgRNA. And at the 3’ end, the target-16s region pairs with the targeted B.cereus 16srRNA sequence and enables cleavage.