Difference between revisions of "Part:BBa K4368004"

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<partinfo>BBa_K4368004 short</partinfo>
 
<partinfo>BBa_K4368004 short</partinfo>
  
''GlgC16'' encodes for the ADP-glucose pyrophosphorylase gene of ''Escherichia coli'' ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K118016 BBa_K118016]). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) as well as a promoter inducible by the cI protein of the phage lambda (BBa_R1051).
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''GlgC16'' encodes for the ADP-glucose pyrophosphorylase gene of ''Escherichia coli'' ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K118016 BBa_K118016]). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015] BBa_B0015) as well as a promoter inducible by the cI protein of the phage lambda (BBa_R1051).
 
The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.
 
The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.
  

Revision as of 16:37, 4 October 2022


pcI + rbs + glgC16 + terminator

GlgC16 encodes for the ADP-glucose pyrophosphorylase gene of Escherichia coli (BBa_K118016). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator ([1] BBa_B0015) as well as a promoter inducible by the cI protein of the phage lambda (BBa_R1051). The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization from iGEM22_UMA_MALAGA