Difference between revisions of "Part:BBa K4115014"
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===Usage and Biology=== | ===Usage and Biology=== | ||
The nitrogen-fixing gene cluster comes into play due to the downstream protein expression of PnifH in the original A. caul genome[1]. The PnifH fragment was added to our designed system and sfGFP(<partinfo>BBa_K4115000</partinfo>) was expressed downstream to verify whether the artificially designed system could regulate nif gene cluster as expected in A. caul.<br> | The nitrogen-fixing gene cluster comes into play due to the downstream protein expression of PnifH in the original A. caul genome[1]. The PnifH fragment was added to our designed system and sfGFP(<partinfo>BBa_K4115000</partinfo>) was expressed downstream to verify whether the artificially designed system could regulate nif gene cluster as expected in A. caul.<br> | ||
| − | [1]Ryu, MH., Zhang, J., Toth, T. et al. Control of nitrogen fixation in bacteria that associate with cereals. Nat Microbiol 5, 314–330 (2020). https://doi.org/10.1038/s41564-019-0631-2 | + | [1]Ryu, MH., Zhang, J., Toth, T. et al. Control of nitrogen fixation in bacteria that associate with cereals. Nat Microbiol 5, 314–330 (2020). https://doi.org/10.1038/s41564-019-0631-2<br> |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4115014 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4115014 SequenceAndFeatures</partinfo> | ||
Revision as of 14:28, 4 October 2022
PnifH（with RBS）
The promoter of the nifH coding sequence on the nitrogen-fixing gene cluster is regulated by the nifA protein.
Usage and Biology
The nitrogen-fixing gene cluster comes into play due to the downstream protein expression of PnifH in the original A. caul genome[1]. The PnifH fragment was added to our designed system and sfGFP(BBa_K4115000) was expressed downstream to verify whether the artificially designed system could regulate nif gene cluster as expected in A. caul.
[1]Ryu, MH., Zhang, J., Toth, T. et al. Control of nitrogen fixation in bacteria that associate with cereals. Nat Microbiol 5, 314–330 (2020). https://doi.org/10.1038/s41564-019-0631-2
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 272