Difference between revisions of "Part:BBa K4387005"
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===Usage and Biology===
 
===Usage and Biology===
  
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This composite part consists of the inducible pNorVβ promoter (<html><a href="https://parts.igem.org/Part:BBa_K4387000">BBa_K4387000</a></html>), superfolder GFP preceded by one strong ribosomal binding site (<html><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></html>, <html><a href="https://parts.igem.org/Part:BBa_K2553008">BBa_K2553008</a></html>), the NorR regulator (<html><a href="https://parts.igem.org/Part:BBa_K4387001">BBa_K4387001</a></html>), and a double forward (<html><a href="https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>). We chose a high-copy backbone from Twist for this part. Due to the competitive binding of the activated and inactivated NorR on the promoter, we decided on this construct with a positive feedback loop that adjusted the levels of NorR. The presence of nitric oxide would activate PnorV_beta to induce GFP and NorR expression. Thereby, we ensure that high amounts of NorR will be produced in the presence of NO and in the presence of NO only.   
+
This composite part consists of the inducible pNorVβ promoter (<html><a href="https://parts.igem.org/Part:BBa_K4387000">BBa_K4387000</a></html>), superfolder GFP preceded by one strong ribosomal binding site (<html><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></html>, <html><a href="https://parts.igem.org/Part:BBa_K2553008">BBa_K2553008</a></html>), the NorR regulator (<html><a href="https://parts.igem.org/Part:BBa_K4387001">BBa_K4387001</a></html>), and a double forward (<html><a href="https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>). We chose a high-copy backbone from Twist for this part. Due to the competitive binding of the activated and inactivated NorR on the promoter, we decided on this construct with a positive feedback loop that adjusted the levels of NorR. The presence of nitric oxide would activate pNorVβ to induce GFP and NorR expression. Thereby, we ensure that high amounts of NorR will be produced in the presence of NO and in the presence of NO only.   
  
 
This construct was tested in the bacterial strain E.coli Nissle 1917.
 
This construct was tested in the bacterial strain E.coli Nissle 1917.

Revision as of 12:21, 4 October 2022


Nitric Oxide Sensing Genetic Circuit With One Ribosomal Binding Site

Usage and Biology

This composite part consists of the inducible pNorVβ promoter (BBa_K4387000), superfolder GFP preceded by one strong ribosomal binding site (BBa_B0034, BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward (BBa_B0015). We chose a high-copy backbone from Twist for this part. Due to the competitive binding of the activated and inactivated NorR on the promoter, we decided on this construct with a positive feedback loop that adjusted the levels of NorR. The presence of nitric oxide would activate pNorVβ to induce GFP and NorR expression. Thereby, we ensure that high amounts of NorR will be produced in the presence of NO and in the presence of NO only.

This construct was tested in the bacterial strain E.coli Nissle 1917.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 703
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Measurements

Figure 1: Induction response of the pNorVβ promoter to different DETA/NO concentrations with respect to time.
Figure 2: Dose response of the pNorVβ promoter at different DETA/NO concentrations.




References

Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223