Difference between revisions of "Part:BBa K4387004"
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===Usage and Biology===
 
===Usage and Biology===
  
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This composite part consists of the ETH promoter NorV (<html><a href="https://parts.igem.org/Part:BBa_K2116002">BBa_K2116002</a></html>), superfolder GFP preceded by one strong ribosomal binding site (<html><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></html>, <html><a href="https://parts.igem.org/Part:BBa_K2553008">BBa_K2553008</a></html>), the NorR regulator (<html><a href="https://parts.igem.org/Part:BBa_K4387001">BBa_K4387001</a></html>), and a double forward (<html><a href="https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>). We wanted to compare this ETH NorV promoter to the pNorVβ promoter and see which one was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team when tested at different concentration levels.
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This composite part consists of the ETH promoter NorV (<html><a href="https://parts.igem.org/Part:BBa_K2116002">BBa_K2116002</a></html>), superfolder GFP preceded by one strong ribosomal binding site (<html><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></html>, <html><a href="https://parts.igem.org/Part:BBa_K2553008">BBa_K2553008</a></html>), the NorR regulator (<html><a href="https://parts.igem.org/Part:BBa_K4387001">BBa_K4387001</a></html>), and a double forward (<html><a href="https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>). We chose a high-copy backbone from Twist for this part. We wanted to compare this ETH NorV promoter to the pNorVβ promoter and see which one was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team when tested at different concentration levels.
  
 
This construct was tested in the bacterial strain E.coli Nissle 1917.
 
This construct was tested in the bacterial strain E.coli Nissle 1917.

Revision as of 12:12, 4 October 2022


Nitric Oxide Sensing Genetic Circuit with Promoter BBa_K2116002

Usage and Biology

This composite part consists of the ETH promoter NorV (BBa_K2116002), superfolder GFP preceded by one strong ribosomal binding site (BBa_B0034, BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward (BBa_B0015). We chose a high-copy backbone from Twist for this part. We wanted to compare this ETH NorV promoter to the pNorVβ promoter and see which one was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team when tested at different concentration levels.

This construct was tested in the bacterial strain E.coli Nissle 1917.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 742
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Measurements

Figure 1: Induction response of the 2016 ETH Team NorV promoter to different DETA/NO concentrations with respect to time.
Figure 2: Dose response of the 2016 ETH Team NorV promoter at different DETA/NO concentrations.