Difference between revisions of "Part:BBa K4115007"
 
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This is a stable sequence located upstream of the nifA sequence in the genome of Azorhizobium caulinodans ORS571. It is used as a one-sided homology arm on the designed plasmid to help recombine knockout of nifA on its original genome and introduce the designed regulatory characterization sequence.[1]
 
This is a stable sequence located upstream of the nifA sequence in the genome of Azorhizobium caulinodans ORS571. It is used as a one-sided homology arm on the designed plasmid to help recombine knockout of nifA on its original genome and introduce the designed regulatory characterization sequence.[1]
 
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===Usage and Biology===
 
===Usage and Biology===
 
Detailed applications and related experiments can be found in <partinfo>BBa_K4115002</partinfo> <partinfo>BBa_K4115041</partinfo>
 
Detailed applications and related experiments can be found in <partinfo>BBa_K4115002</partinfo> <partinfo>BBa_K4115041</partinfo>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4115007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4115007 SequenceAndFeatures</partinfo>

Latest revision as of 10:56, 4 October 2022


NifA_upstream

This is a stable sequence located upstream of the nifA sequence in the genome of Azorhizobium caulinodans ORS571. It is used as a one-sided homology arm on the designed plasmid to help recombine knockout of nifA on its original genome and introduce the designed regulatory characterization sequence.[1]

Usage and Biology

Detailed applications and related experiments can be found in BBa_K4115002 BBa_K4115041

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 434
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 547
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 234
    Illegal NgoMIV site found at 339
    Illegal NgoMIV site found at 556
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 331

[1]Ryu, M. H., Zhang, J., Toth, T., Khokhani, D., Geddes, B. A., Mus, F., Garcia-Costas, A., Peters, J. W., Poole, P. S., Ané, J. M., & Voigt, C. A. (2020). Control of nitrogen fixation in bacteria that associate with cereals. Nature microbiology, 5(2), 314–330. https://doi.org/10.1038/s41564-019-0631-2