Difference between revisions of "Part:BBa K4387004"
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This composite part consists of the ETH promoter NorV (<html><a href="http://https://parts.igem.org/Part:BBa_K2116002">BBa_K2116002</a></html>), a superfolder GFP (<html><a href="http://https://parts.igem.org/Part:BBa_K2553008">BBa_K2553008</a></html>), the NorR regulator (<html><a href="http://https://parts.igem.org/Part:BBa_K4387001">BBa_K4387001</a></html>), and a double forward terminator (<html><a href="http://https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>). We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team. | This composite part consists of the ETH promoter NorV (<html><a href="http://https://parts.igem.org/Part:BBa_K2116002">BBa_K2116002</a></html>), a superfolder GFP (<html><a href="http://https://parts.igem.org/Part:BBa_K2553008">BBa_K2553008</a></html>), the NorR regulator (<html><a href="http://https://parts.igem.org/Part:BBa_K4387001">BBa_K4387001</a></html>), and a double forward terminator (<html><a href="http://https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>). We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team. | ||
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This construct was tested in the bacterial strain E.coli Nissle 1917. | This construct was tested in the bacterial strain E.coli Nissle 1917. | ||
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<partinfo>BBa_K4387004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4387004 SequenceAndFeatures</partinfo> | ||
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| − | [[File:ResponseETH.jpeg|450px|thumb|right|'''Figure 2:''' Dose | + | [[File:ResponseETH.jpeg|450px|thumb|right|'''Figure 2:''' Dose response of the 2016 ETH Team NorV promoter at different DETA/NO concentrations.]] |
Revision as of 10:38, 4 October 2022
Nitric Oxide Sensing Genetic Circuit with Promoter BBa_K2116002
Usage and Biology
This composite part consists of the ETH promoter NorV (BBa_K2116002), a superfolder GFP (BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward terminator (BBa_B0015). We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team.
This construct was tested in the bacterial strain E.coli Nissle 1917.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 742
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
Results
TYPE HERE
