Difference between revisions of "Part:BBa K4387000"
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===Usage and Biology===
 
===Usage and Biology===
  
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pNorVβ is an optimized nitric oxide sensitive promoter. In our constructs, this promoter is coupled to sfGFP and NorR. The corresponding integration host factor IHF2 binding site was removed from the promoter pNorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used nitric oxide source in the experiment, activating the downstream genes' transcription[1].
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The inducible pNorVβ is an optimized nitric oxide sensitive promoter. In our constructs, this promoter is coupled to sfGFP and NorR. The corresponding integration host factor IHF2 binding site was removed from the promoter pNorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used nitric oxide source in the experiment, activating the downstream genes' transcription[1].
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This promoter was tested in the bacterial strain E.coli Nissle 1917, and it was successfully coupled with other iGEM parts in the following constructs:
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<ul>
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<li>Nitric Oxide Sensing Genetic Circuit With One Ribosomal Binding Site [https://parts.igem.org/Part:BBa_K4387005 (BBa_K4387005)]</li>
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<li>Nitric Oxide Sensing Genetic Circuit With Two Ribosomal Binding Site [https://parts.igem.org/Part:BBa_K4387006 (BBa_K4387006)]</li>
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<li>Nitric Oxide Sensing Genetic Circuit With Three Ribosomal Binding Site [https://parts.igem.org/Part:BBa_K4387007 (BBa_K4387007)]</li>
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<li>Nitric Oxide Sensing Genetic Circuit With Two Ribosomal Binding Site and Without [https://parts.igem.org/Part:BBa_K4387001 (BBa_K4387001)] [https://parts.igem.org/Part:BBa_K4387009 (BBa_K4387009)]</li>
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</ul>
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This promoter was tested in the bacterial strain E.coli Nissle 1917.
 
  
  
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<partinfo>BBa_K4387000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4387000 SequenceAndFeatures</partinfo>
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Revision as of 10:14, 4 October 2022


Nitric Oxide Sensing Promoter pNorVβ

Usage and Biology

The inducible pNorVβ is an optimized nitric oxide sensitive promoter. In our constructs, this promoter is coupled to sfGFP and NorR. The corresponding integration host factor IHF2 binding site was removed from the promoter pNorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used nitric oxide source in the experiment, activating the downstream genes' transcription[1].

This promoter was tested in the bacterial strain E.coli Nissle 1917, and it was successfully coupled with other iGEM parts in the following constructs:

  • Nitric Oxide Sensing Genetic Circuit With One Ribosomal Binding Site (BBa_K4387005)
  • Nitric Oxide Sensing Genetic Circuit With Two Ribosomal Binding Site (BBa_K4387006)
  • Nitric Oxide Sensing Genetic Circuit With Three Ribosomal Binding Site (BBa_K4387007)
  • Nitric Oxide Sensing Genetic Circuit With Two Ribosomal Binding Site and Without (BBa_K4387001) (BBa_K4387009)



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223