Difference between revisions of "Part:BBa K4387000"
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<partinfo>BBa_K4387000 short</partinfo> | <partinfo>BBa_K4387000 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | pNorVβ is an optimized nitric oxide sensitive promoter. In our constructs, this promoter is coupled to sfGFP and NorR. The corresponding integration host factor IHF2 binding site was removed from the promoter pNorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used nitric oxide source in the experiment, activating the downstream genes' transcription[1]. | |
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+ | This promoter was tested in the bacterial strain E.coli Nissle 1917. | ||
+ | ===Sequence and Features=== | ||
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− | <span class='h3bb' | + | <span class='h3bb'/span> |
<partinfo>BBa_K4387000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4387000 SequenceAndFeatures</partinfo> | ||
Revision as of 09:44, 4 October 2022
Nitric Oxide Sensing Promoter pNorVβ
Usage and Biology
pNorVβ is an optimized nitric oxide sensitive promoter. In our constructs, this promoter is coupled to sfGFP and NorR. The corresponding integration host factor IHF2 binding site was removed from the promoter pNorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used nitric oxide source in the experiment, activating the downstream genes' transcription[1].
This promoter was tested in the bacterial strain E.coli Nissle 1917.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]