Difference between revisions of "Part:BBa K2205000"
Svetlana ia (Talk | contribs) |
Svetlana ia (Talk | contribs) |
||
| Line 9: | Line 9: | ||
===Part Documentation by TU_Dresden 2022 Team=== | ===Part Documentation by TU_Dresden 2022 Team=== | ||
| − | Part was compared to | + | Part was compared to [https://parts.igem.org/Part:BBa_J04450 BBa_J04450]. |
| + | |||
| + | [[File:Muc16 SignalPeptideprediction_002.png|thumb|center|850px| | ||
| + | |||
| + | '''Figure 1''': Test description]] | ||
Revision as of 08:50, 4 October 2022
J23100-RFP
BBa_K2205000 is a proposed composite BioBrick reporter that constitutively produces MFRP1. It possesses an MRFP1 coding sequence (E1010) after a strong Anderson promoter (J23100) and RBS (B0034). A double terminator (B0015) was added after the MRFP1 sequence. A BioBrick RFC10 prefix and suffix were added as well.
The construct was submitted to IDT for synthesis as a gBlock. Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.
Part Documentation by TU_Dresden 2022 Team
Part was compared to BBa_J04450.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 616
Illegal AgeI site found at 728 - 1000COMPATIBLE WITH RFC[1000]