Difference between revisions of "Part:BBa K2205000"

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The construct was submitted to IDT for synthesis as a gBlock.
 
The construct was submitted to IDT for synthesis as a gBlock.
 
Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.
 
Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.
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===Part Documentation by TU_Dresden 2022 Team===
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Part was compared to <a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a>.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2205000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2205000 SequenceAndFeatures</partinfo>

Revision as of 08:48, 4 October 2022


J23100-RFP

BBa_K2205000 is a proposed composite BioBrick reporter that constitutively produces MFRP1. It possesses an MRFP1 coding sequence (E1010) after a strong Anderson promoter (J23100) and RBS (B0034). A double terminator (B0015) was added after the MRFP1 sequence. A BioBrick RFC10 prefix and suffix were added as well.

The construct was submitted to IDT for synthesis as a gBlock. Due to time constraints, work on assembling and further characterising this Biobrick was unable to finish.

Part Documentation by TU_Dresden 2022 Team

Part was compared to <a href="https://parts.igem.org/Part:BBa_J04450">BBa_J04450</a>.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]