Difference between revisions of "Part:BBa K4271001"

Revision as of 08:01, 4 October 2022

T7 Promoter + Lac operator + RBS + OPH + T7 terminator

This sequence is responsible for the regulation and expression of the OPH gene. The T7 promoter, transcribed by only the T7 RNA polymerase, identifies the sequence downstream and enables fast and effective transcription. We further designed a lac operon, so that upon IPTG induction, the lacI repressor protein will be detached from the lacI gene, leading to the transcription and translation of our target oph gene. The ribosome binding site (RBS) is where the ribosome bind on the mRNA for translation. This RBS is taken from the pET22B vector. OPH a gene that encodes organophosphate hydrolase, paraoxon, a type of organic phosphate and insecticide. The product of the paraoxon degradation, pNP, will be detected to evaluate the efficacy of the gene. The T7 terminator identifies the end of the transcription sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1259
Illegal NotI site found at 1219
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1228
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 330
Illegal AgeI site found at 150
Illegal AgeI site found at 435
Illegal AgeI site found at 570
Illegal AgeI site found at 633
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4271001 short</partinfo>
-This sequence is responsible for the regulation and expression of the OPH gene. The T7 promoter, transcribed by only the T7 RNA polymerase, identifies the sequence downstream and enables fast and effective transcription. The Lac operator is a repressor, hence prohibiting the expression of the OPH gene. Therefore, we use IPTG as an inducer to initiate transcription. The ribosome binding site (RBS) is where the ribosome bind on the mRNA for translation. This RBS is taken from the pET22B vector. OPH a gene that encodes organophosphate hydrolase, paraoxon, a type of organic phosphate and insecticide. The product of the paraoxon degradation, pNP, will be detected to evaluate the efficacy of the gene. The T7 terminator identifies the end of the transcription sequence.
+This sequence is responsible for the regulation and expression of the OPH gene. The T7 promoter, transcribed by only the T7 RNA polymerase, identifies the sequence downstream and enables fast and effective transcription. We further designed a lac operon, so that upon IPTG induction, the lacI repressor protein will be detached from the lacI gene, leading to the transcription and translation of our target oph gene. The ribosome binding site (RBS) is where the ribosome bind on the mRNA for translation. This RBS is taken from the pET22B vector. OPH a gene that encodes organophosphate hydrolase, paraoxon, a type of organic phosphate and insecticide. The product of the paraoxon degradation, pNP, will be detected to evaluate the efficacy of the gene. The T7 terminator identifies the end of the transcription sequence.
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