Difference between revisions of "Part:BBa K4271001"

Revision as of 07:43, 4 October 2022

T7 Promoter + Lac operator + RBS + OPH + T7 terminator

This sequence is responsible for the regulation and expression of the OPH gene. The T7 promoter, transcribed by only the T7 RNA polymerase, identifies the sequence downstream and enables fast and effective transcription. The Lac operator is a repressor, hence prohibiting the expression of the OPH gene. Therefore, we use IPTG as an inducer to initiate transcription. The ribosome binding site (RBS) is where the ribosome bind on the mRNA for translation. This RBS is taken from the pET22B vector. OPH a gene that encodes organophosphate hydrolase, paraoxon, a type of organic phosphate and insecticide. The product of the paraoxon degradation, pNP, will be detected to evaluate the efficacy of the gene. The T7 terminator identifies the end of the transcription sequence.

Sequence and Features

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