Difference between revisions of "Part:BBa K4399012:Design"
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===Design Notes===
 
===Design Notes===
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In anthocyanin biosynthesis study, we need a negative control. We constructed the part without an XVE (BBa_P10300) gene, so SbMYB75 (BBa_K4399003) and SbDEL (BBa_K4399004) can’t be expressed. So anthocyanin biosynthesis can’t be switched on.
+
Constitutively expressed XVE (BBa_P10300) transcription factor could be activated in presence of β-estrogen, then entry cell nucleus, bind to LexA-35S (BBa_K3900024) promoter, thus activate expression of SbMYB75 (BBa_K4399003) and SbDEL (BBa_K4399004). The two transcription factor can switch on endogenous structural genes for anthocyanins biosynthesis. The constitutive GFP (BBa_K4399002) expression box was employed to facilitate screening of transformants.
  
  
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===Source===
 
  
−
The sequence is based on BBa_K4399008, BBa_K4399009 and BBa_K4399010.
+
===Source===
  
 +
The sequence is based on BBa_K4399008, BBa_K4399009, BBa_K4399010 and BBa_K4399011.
  
 
===References===
 
===References===

Revision as of 07:36, 4 October 2022


The negative control for anthocyanin biosynthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 5513
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
    Illegal PstI site found at 2759
    Illegal PstI site found at 4179
    Illegal PstI site found at 4264
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 5513
    Illegal NheI site found at 1244
    Illegal PstI site found at 2759
    Illegal PstI site found at 4179
    Illegal PstI site found at 4264
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 5513
    Illegal BglII site found at 4113
    Illegal BglII site found at 4925
    Illegal BamHI site found at 3547
    Illegal BamHI site found at 3849
    Illegal BamHI site found at 5168
    Illegal BamHI site found at 5237
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 5513
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
    Illegal PstI site found at 2759
    Illegal PstI site found at 4179
    Illegal PstI site found at 4264
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 5513
    Illegal XbaI site found at 696
    Illegal XbaI site found at 1579
    Illegal PstI site found at 2759
    Illegal PstI site found at 4179
    Illegal PstI site found at 4264
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constitutively expressed XVE (BBa_P10300) transcription factor could be activated in presence of β-estrogen, then entry cell nucleus, bind to LexA-35S (BBa_K3900024) promoter, thus activate expression of SbMYB75 (BBa_K4399003) and SbDEL (BBa_K4399004). The two transcription factor can switch on endogenous structural genes for anthocyanins biosynthesis. The constitutive GFP (BBa_K4399002) expression box was employed to facilitate screening of transformants.


Source

The sequence is based on BBa_K4399008, BBa_K4399009, BBa_K4399010 and BBa_K4399011.

References