Difference between revisions of "Part:BBa K3570009"
 
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<h2>Usage</h2>
 
<h2>Usage</h2>
 
HO upstream homology arm part shall be used together with HO downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570010 BBa_K3570010]) to target a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae'</i>s genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.
 
HO upstream homology arm part shall be used together with HO downstream homology arm part ([https://parts.igem.org/Part:BBa_K3570010 BBa_K3570010]) to target a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the <i>S. cerevisiae'</i>s genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.
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This sequence was identified from a personal communication with Dr. Gilles Truan. </p>
  
 
<h2>Experiments</h2>
 
<h2>Experiments</h2>
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3570009 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4263009 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3570009 parameters</partinfo>
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<partinfo>BBa_K4263009 parameters</partinfo>
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Latest revision as of 06:52, 4 October 2022


HO upstream homologous sequence

Usage

HO upstream homology arm part shall be used together with HO downstream homology arm part (BBa_K3570010) to target a functional yeast integration locus. When HO upstream put to 5' of the biobrick together with HO downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Homothallic switching endonuclease (HO) gene.

This sequence was identified from a personal communication with Dr. Gilles Truan.

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References

  • [1]S. cerevisiae genome, chromosome IV, HO gene. GenBank: CP046084.1