Difference between revisions of "Part:BBa K4206002"
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<partinfo>BBa_K4206002 short</partinfo> | <partinfo>BBa_K4206002 short</partinfo> | ||
− | This part | + | This part contains of the coding sequences of endo-1,4-beta-glucanases, exo-1,4-beta-glucanases and beta-glucosidases with PhoA signal sequence. |
− | The complete cellulose hydrolysis reaction proceeds by these enzymes. | + | The complete cellulose hydrolysis reaction proceeds by these enzymes in the downstream of constitutive promoter. |
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Revision as of 04:57, 4 October 2022
cellulases with constitutive promoter
This part contains of the coding sequences of endo-1,4-beta-glucanases, exo-1,4-beta-glucanases and beta-glucosidases with PhoA signal sequence. The complete cellulose hydrolysis reaction proceeds by these enzymes in the downstream of constitutive promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 4717 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4350
Illegal NgoMIV site found at 4723
Illegal NgoMIV site found at 5225
Illegal AgeI site found at 378
Illegal AgeI site found at 1842 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4770
Illegal SapI.rc site found at 4853