Difference between revisions of "Part:BBa K4115009:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | |||
+ | NS3-2 and NSI-3-1 are a pair of commonly used homology arms in <i>S.elongatus</i>. | ||
===Source=== | ===Source=== | ||
− | + | The sequence of NS3-2 is gained from a NS3-targeting plasmid [1] and the fragment we use is artificially synthesized by the company. | |
===References=== | ===References=== | ||
+ | [1] Lin PC, Zhang F, Pakrasi HB. Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Sci Rep. 2020 Jan 15;10(1):390. doi: 10.1038/s41598-019-57319-5. PMID: 31942010; PMCID: PMC6962321. |
Latest revision as of 02:39, 4 October 2022
NS3-2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 507
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 507
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 507
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 507
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
NS3-2 and NSI-3-1 are a pair of commonly used homology arms in S.elongatus.
Source
The sequence of NS3-2 is gained from a NS3-targeting plasmid [1] and the fragment we use is artificially synthesized by the company.
References
[1] Lin PC, Zhang F, Pakrasi HB. Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Sci Rep. 2020 Jan 15;10(1):390. doi: 10.1038/s41598-019-57319-5. PMID: 31942010; PMCID: PMC6962321.