Difference between revisions of "Part:BBa K4115011:Design"
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
NSI-DS is placed downstream of the aimed fragments when constructing our plasmids needed for HL7942 and UTEX2973.
 
  
 +
NSI-US and NSI-DS are a pair of commonly used homology arms in S.elongatus.
  
  
 
===Source===
 
===Source===
  
−
The sequence is gained from the genome of Synechococcus 2973 and the fragment we use is synthesized by the company.
+
The sequence is gained from the genome of Synechococcus 2973 [1] and the fragment we use is synthesized by the company.
  
 
===References===
 
===References===
 +
[1] Li, S., Sun, T., Chen, L., Zhang, W. (2021). Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria. In: Basu, C. (eds) Biofuels and Biodiesel. Methods in Molecular Biology, vol 2290. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1323-8_16

Revision as of 02:26, 4 October 2022


NSI-DS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 442
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 442
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 442
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 442
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NSI-US and NSI-DS are a pair of commonly used homology arms in S.elongatus.


Source

The sequence is gained from the genome of Synechococcus 2973 [1] and the fragment we use is synthesized by the company.

References

[1] Li, S., Sun, T., Chen, L., Zhang, W. (2021). Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria. In: Basu, C. (eds) Biofuels and Biodiesel. Methods in Molecular Biology, vol 2290. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1323-8_16