Difference between revisions of "Part:BBa K4115010"

 
 
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<partinfo>BBa_K4115010 short</partinfo>
 
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An upstreaming homology arm, which is used together with NSI-DS to construct plasmids required for Synechococcus.
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An upstream homology arm from <i>Synechococcus</i> 2973.
  
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===Usage and Biology===
 
===Usage and Biology===
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It is used together with NSI-DS [https://parts.igem.org/Part:BBa_K4115011 (BBa_K4115011)] to construct plasmids required for <i>S.elongatus</i> HL7942, where NSI-US is placed upstream of the aimed fragments.
  
 
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Latest revision as of 02:22, 4 October 2022


NSI-US

An upstream homology arm from Synechococcus 2973.

Usage and Biology

It is used together with NSI-DS (BBa_K4115011) to construct plasmids required for S.elongatus HL7942, where NSI-US is placed upstream of the aimed fragments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 333
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 520
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 333
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 333
  • 1000
    COMPATIBLE WITH RFC[1000]