Difference between revisions of "Part:BBa K4115010:Design"
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
NSI-US is placed upstream of the aimed fragments when constructing our plasmids needed for HL7942 and UTEX2973.
 
  
 +
NSI-US and NSI-DS is a pair of commonly used homology arms in <i>S.elongatus</i>.
  
  
 
===Source===
 
===Source===
  
−
The sequence is gained from the genome of Synechococcus 2973 and the fragment we use is synthesized by the company.
+
The sequence is gained from the genome of <i>Synechococcus</i> 2973 and the fragment we use is synthesized by the company.
  
 
===References===
 
===References===

Revision as of 02:18, 4 October 2022


NSI-US


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 333
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 520
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 333
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 333
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NSI-US and NSI-DS is a pair of commonly used homology arms in S.elongatus.


Source

The sequence is gained from the genome of Synechococcus 2973 and the fragment we use is synthesized by the company.

References