Difference between revisions of "Part:BBa K4387004"
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===Results===
 
===Results===
  
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[[File:DoseResponseETH.jpeg|450px|thumb|right|'''Figure 1:''' Induction response of the 2016 ETH Team NorV promoter to different DETA/NO concentrations with respect to time.]]
  
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[[File:ResponseETH.jpeg|450px|thumb|right|'''Figure 2:''' Dose Response of the 2016 ETH Team NorV Promoter at different DETA/NO concentrations.]]
  
  

Revision as of 23:27, 3 October 2022


Nitric Oxide Sensing Genetic Circuit with Promoter BBa_K2116002

Usage and Biology

This composite part consists of the ETH promoter NorV (BBa_K2116002), a superfolder GFP (BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward terminator (BBa_B0015). We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team.


This construct was tested in the bacterial strain E.coli Nissle 1917.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 742
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Results

Figure 1: Induction response of the 2016 ETH Team NorV promoter to different DETA/NO concentrations with respect to time.

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Figure 2: Dose Response of the 2016 ETH Team NorV Promoter at different DETA/NO concentrations.