Difference between revisions of "Part:BBa K4115023:Design"

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===Design Notes===
 
===Design Notes===
−
Designed for constitutively expression of sf-GFP protein in E. coli.
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Designed for constitutively expression of sf-GFP protein with degradation tag in E. coli.
 +
After being transfected with this part, the E.ocli will have a constitutive green fluorescence.
  
 
===Source===
 
===Source===

Latest revision as of 13:53, 3 October 2022


J23101-B0034-sfGFP(with LVA)-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed for constitutively expression of sf-GFP protein with degradation tag in E. coli. After being transfected with this part, the E.ocli will have a constitutive green fluorescence.

Source

Obtained by assembling the corresponding parts in sequence by Gibson Assembly.

References