Difference between revisions of "Part:BBa K4115017:Design"

 
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===Design Notes===
 
===Design Notes===
 
SacC gene can enhance the ability of Escherichia coli to utilize environmental sucrose.
 
SacC gene can enhance the ability of Escherichia coli to utilize environmental sucrose.
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Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.[1]
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At the same time, the introduction of SacC will cause E. coli to produce a large amount of organic acids, which is exactly the organic carbon source preferred by nitrogen-fixing bacteria (A.caul). The intersection of nutritional structures will help the entire symbiotic system to be more stable and efficient.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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[1] Lee, J.W., Choi, S., Park, J.H., Vickers, C.E., Nielsen, L.K., and Lee, S.Y. (2010). Development of sucrose-utilizing Escherichia coli K-12 strain by cloning beta-fructofuranosidases and its application for L-threonine production. Appl Microbiol Biotechnol 88, 905-913. 10.1007/s00253-010-2825-7.

Latest revision as of 09:06, 3 October 2022


SacC (the extracellular sucrase gene)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 437
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 437
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SacC gene can enhance the ability of Escherichia coli to utilize environmental sucrose. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.[1] At the same time, the introduction of SacC will cause E. coli to produce a large amount of organic acids, which is exactly the organic carbon source preferred by nitrogen-fixing bacteria (A.caul). The intersection of nutritional structures will help the entire symbiotic system to be more stable and efficient.

Source

The SacC gene was originally found in the Zymomonas mobilis genome. We obtain gene fragments through commercial synthesis companies.

References

[1] Lee, J.W., Choi, S., Park, J.H., Vickers, C.E., Nielsen, L.K., and Lee, S.Y. (2010). Development of sucrose-utilizing Escherichia coli K-12 strain by cloning beta-fructofuranosidases and its application for L-threonine production. Appl Microbiol Biotechnol 88, 905-913. 10.1007/s00253-010-2825-7.