Difference between revisions of "Part:BBa K4115017"
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The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics. | The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics. | ||
− | [[Image:SacCgrowthcurve1.png|450px|thumb|left|'''Figure 1:''' Fermentation of ''E. coli'' | + | [[Image:SacCgrowthcurve1.png|450px|thumb|left|'''Figure 1:''' Fermentation of ''E. coli'' with <partinfo>BBa_K4115017</partinfo> in 300ml conical flask in biological shaker, scale: 100 mL M9 medium, 37 °C, 220 rpm, initial pH 7.5, OD<sub>600</sub> measured every 2 hours.]] |
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Revision as of 06:57, 3 October 2022
SacC (the extracellular sucrase gene)
The sequence of Zymomonas mobilis gene sacC that encodes the extracellular sucrase. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 437
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1309
Illegal PstI site found at 437 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1352
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 437
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 437
Illegal NgoMIV site found at 696
Illegal AgeI site found at 418 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.
![](/wiki/images/d/dc/SacCgrowthcurve1.png)
Figure 1: Fermentation of E. coli with BBa_K4115017 in 300ml conical flask in biological shaker, scale: 100 mL M9 medium, 37 °C, 220 rpm, initial pH 7.5, OD600 measured every 2 hours.