Difference between revisions of "Part:BBa K4115017"
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<partinfo>BBa_K4115017 short</partinfo> | <partinfo>BBa_K4115017 short</partinfo> | ||
− | The sequence of Zymomonas mobilis gene sacC that encodes the extracellular sucrase. | + | The sequence of Zymomonas mobilis gene sacC that encodes the extracellular sucrase. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4115017 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4115017 SequenceAndFeatures</partinfo> | ||
− | + | The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics. | |
+ | [[Image:SacCgrowthcurve1.png|450px|thumb|left|'''Figure 1:''' Fermentation of ''E. coli'' KRXwith <partinfo>BBa_K863005</partinfo> (ECOL) in an Infors Labfors Bioreactor, scale: 3 L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold pO<sub>2</sub> at 50 %, OD<sub>600</sub> measured every 30 minutes.]] | ||
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Revision as of 06:35, 3 October 2022
SacC (the extracellular sucrase gene)
The sequence of Zymomonas mobilis gene sacC that encodes the extracellular sucrase.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 437
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1309
Illegal PstI site found at 437 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1352
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 437
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 437
Illegal NgoMIV site found at 696
Illegal AgeI site found at 418 - 1000COMPATIBLE WITH RFC[1000]
The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.
![](/wiki/images/d/dc/SacCgrowthcurve1.png)
Figure 1: Fermentation of E. coli KRXwith BBa_K863005 (ECOL) in an Infors Labfors Bioreactor, scale: 3 L, [http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction medium] + 60 µg/mL chloramphenicol, 37 °C, pH 7, agitation on cascade to hold pO2 at 50 %, OD600 measured every 30 minutes.