Difference between revisions of "Part:BBa K4115045"

Revision as of 05:54, 3 October 2022

NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1

This composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. The core component of this composite part is cscB(BBa_K3971011), a membrane transporter that transports sucrose to the outside of the cell with the help of the H gradient. We engineered cscB into HL7942 so that it can export sucrose to be used as the carbon source for E. coli.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 507
Illegal PstI site found at 4793
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 507
Illegal PstI site found at 4793
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 507
Illegal PstI site found at 4793
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 507
Illegal PstI site found at 4793
Illegal NgoMIV site found at 1678
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1722

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 This composite part is integrated into the plasmid backbone of PUC57 and entered Synechococcus HL7942 by natural transformation with E. coli. The core component of this composite part is cscB(BBa_K3971011), a membrane transporter that transports sucrose to the outside of the cell with the help of the H gradient. We engineered cscB into HL7942 so that it can export sucrose to be used as the carbon source for E. coli.
-[[File:Overview_of_the_construct_CMV-Cas-VP16_Freigem_2013.png|700px|]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===