Difference between revisions of "Part:BBa K4329003"
 
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===what is it===
 
===what is it===
  
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The quorum sensing suicidal system consists of three parts: LUXR, CRISPRi/dCas9, and sgRNA. The LUXR protein is regulated by the T7 promoter, lac operator, and T7 terminator. CRISPRi/dCas9 is regulated by a quorum sensing promoter and quorum sensing terminator. sgRNA is regulated by the H1 promoter and H1 terminator.  
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BS_United_China introduced this System in 2021(<partinfo>BBa_K4329000</partinfo>). We characterized part of the project to obtain our desired goal: detecting AHL and then activating the CRISPR/Cas9 system.  
  
 
===Usage and Biology===
 
===Usage and Biology===
  
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[[File:Quorum sensing suicidal system.png|700px|thumb|center|Fg.1 The pathway of quorum sensing suicidal system expression]]
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As soon as AHL is detected in the environment, the LuxR promoter will be activated starting the down stream expression of QS promoter and CRISPR/Cas9 system.  
  
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At first, if the IPTG is absent, the RNA ligase in the system will converge with the T7 promoter which can not lead to the production of mRNA. Thus, protein and enzyme production will be inhibited. However, in severe situations, IPTG could be present in the natural environment, so we have considered a double safety system. If LUXR detects the presence of AHL, a quorum sensing chemical sent out by E.Coli, quorum sensing promoter will be activated. Later, the promoter will activate the CRISPRi/dCas9 system targeting the ATP ligase of E.coli. Consequently, without ATP, the bacteria will be forced to die.
 
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===Characterization===
 
  
 
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Latest revision as of 13:13, 2 October 2022


Quorum sensing suicidal system-part1

what is it

BS_United_China introduced this System in 2021(BBa_K4329000). We characterized part of the project to obtain our desired goal: detecting AHL and then activating the CRISPR/Cas9 system.

Usage and Biology

As soon as AHL is detected in the environment, the LuxR promoter will be activated starting the down stream expression of QS promoter and CRISPR/Cas9 system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]