Difference between revisions of "Part:BBa K4329003"
 
(12 intermediate revisions by 2 users not shown)
Line 5: Line 5:
 
===what is it===
 
===what is it===
  
−
The quorum sensing suicidal system consists of three parts: LUXR, CRISPR/Cas9, sgRNA. The LUXR protein is regulated by T7 promoter, lacI operator, and T7 terminator. CRISPR/Cas9 is regulated by a quorum sensing promoter and quorum sensing terminator. sgRNA is regulated by H1 promoter and H1 terminator.  
+
BS_United_China introduced this System in 2021(<partinfo>BBa_K4329000</partinfo>). We characterized part of the project to obtain our desired goal: detecting AHL and then activating the CRISPR/Cas9 system.  
  
−
===what it does===
+
===Usage and Biology===
 +
 
 +
As soon as AHL is detected in the environment, the LuxR promoter will be activated starting the down stream expression of QS promoter and CRISPR/Cas9 system.
  
  

Latest revision as of 13:13, 2 October 2022


Quorum sensing suicidal system-part1

what is it

BS_United_China introduced this System in 2021(BBa_K4329000). We characterized part of the project to obtain our desired goal: detecting AHL and then activating the CRISPR/Cas9 system.

Usage and Biology

As soon as AHL is detected in the environment, the LuxR promoter will be activated starting the down stream expression of QS promoter and CRISPR/Cas9 system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]