Difference between revisions of "Part:BBa K4329002"

m
 
(2 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4329002 short</partinfo>
 
<partinfo>BBa_K4329002 short</partinfo>
  
−
===what is it?===
+
===What is it?===
  
−
CRISPR/dCas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process.  
+
CRISPR/Cas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process.
  
−
===What it does?===
+
===Usage and Biology===
  
 
This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration.  
 
This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
−
===Usage and Biology===
+
 
  
 
<!-- -->
 
<!-- -->

Latest revision as of 11:14, 2 October 2022


sgRNA for targeting Bifunctional BirA enzyme

What is it?

CRISPR/Cas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process.

Usage and Biology

This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 19
  • 1000
    COMPATIBLE WITH RFC[1000]