Difference between revisions of "Part:BBa K4329007"
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<partinfo>BBa_K4329007 short</partinfo> | <partinfo>BBa_K4329007 short</partinfo> | ||
| − | + | ===What is it?=== | |
| + | |||
| + | CRISPR/dCas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process. | ||
| + | |||
| + | ===Usage and Biology=== | ||
| + | |||
| + | This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration. | ||
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Revision as of 11:10, 2 October 2022
sgRNA for targeting ATP synthase in E.coli
What is it?
CRISPR/dCas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process.
Usage and Biology
This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]