Difference between revisions of "Part:BBa K4329003"

Revision as of 10:13, 2 October 2022

Quorum sensing suicidal system-part1

what is it

The quorum sensing suicidal system consists of three parts: LUXR, CRISPR/Cas9, and sgRNA. The LUXR protein is regulated by the T7 promoter, lac operator, and T7 terminator. CRISPR/Cas9 is regulated by a quorum sensing promoter and quorum sensing terminator. sgRNA is regulated by the consensus promoter and terminator.

Usage and Biology

Fg.1 The pathway of quorum sensing suicidal system expression

At first, if the IPTG is absent, the RNA ligase in the system will converge with the T7 promoter which can not lead to the production of mRNA. Thus, protein and enzyme production will be inhibited. However, in severe situations, IPTG could be present in the natural environment, so we have considered a double safety system. If LUXR detects the presence of AHL, a quorum sensing chemical sent out by E.Coli, quorum sensing promoter will be activated. Later, the promoter will activate the CRISPR/Cas9 system targeting the ATP ligase of E.coli. Consequently, without ATP, the bacteria will be forced to die.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===what is it===
-The quorum sensing suicidal system consists of three parts: LUXR, CRISPRi/dCas9, and sgRNA. The LUXR protein is regulated by the T7 promoter, lac operator, and T7 terminator. CRISPRi/dCas9 is regulated by a quorum sensing promoter and quorum sensing terminator. sgRNA is regulated by the H1 promoter and H1 terminator.
+The quorum sensing suicidal system consists of three parts: LUXR, CRISPR/Cas9, and sgRNA. The LUXR protein is regulated by the T7 promoter, lac operator, and T7 terminator. CRISPR/Cas9 is regulated by a quorum sensing promoter and quorum sensing terminator. sgRNA is regulated by the consensus promoter and terminator.
 ===Usage and Biology===
@@ Line 11: / Line 11: @@
 [[File:Quorum sensing suicidal system.png|700px|thumb|center|Fg.1 The pathway of quorum sensing suicidal system expression]]
-At first, if the IPTG is absent, the RNA ligase in the system will converge with the T7 promoter which can not lead to the production of mRNA. Thus, protein and enzyme production will be inhibited. However, in severe situations, IPTG could be present in the natural environment, so we have considered a double safety system. If LUXR detects the presence of AHL, a quorum sensing chemical sent out by E.Coli, quorum sensing promoter will be activated. Later, the promoter will activate the CRISPRi/dCas9 system targeting the ATP ligase of E.coli. Consequently, without ATP, the bacteria will be forced to die.
+At first, if the IPTG is absent, the RNA ligase in the system will converge with the T7 promoter which can not lead to the production of mRNA. Thus, protein and enzyme production will be inhibited. However, in severe situations, IPTG could be present in the natural environment, so we have considered a double safety system. If LUXR detects the presence of AHL, a quorum sensing chemical sent out by E.Coli, quorum sensing promoter will be activated. Later, the promoter will activate the CRISPR/Cas9 system targeting the ATP ligase of E.coli. Consequently, without ATP, the bacteria will be forced to die.
 ===Characterization===