Difference between revisions of "Part:BBa K4180002"

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[[File:BBa K4180002.png|400px|thumb|left|<h3>&emsp;&emsp;&emsp;&emsp;BBa K4180002 <br>&emsp;&emsp;&emsp;&emsp;snf1Δ2-306aa
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[[File:BBa K4180002.png|400px|thumb|left|<h3>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;BBa K4180002 <br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;snf1Δ2-306aa
 
<br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]
 
<br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]
 
[[File:BBa K4180000-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do SNF1 full length PCR - colonies #1, 3, 5, and 6 had BBa_K4180000(SNF1 full length)<br> &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]
 
[[File:BBa K4180000-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do SNF1 full length PCR - colonies #1, 3, 5, and 6 had BBa_K4180000(SNF1 full length)<br> &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]

Revision as of 08:32, 2 October 2022


snf1Δ2-306aa N-truncated

snf1Δ2-306aa, N-terminus truncated from amino acid 2 to 306 deleting kinase domain of SNF1, where the phosphorylation takes place on Thr210 to activate the catalytic activity of SNF1 Sequence and Features

        BBa K4180002
        snf1Δ2-306aa
         PCR product

After Bacteria transformation, bacteria colonies were picking up directly to do SNF1 full length PCR - colonies #1, 3, 5, and 6 had BBa_K4180000(SNF1 full length)
         PCR product



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]