Difference between revisions of "Part:BBa K4115045:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
none
+
 
 +
It is proved that cscB can transport sucrose at a good rate with salt stress and IPTG induced.[1] Thus we choose cscB as an exporter in Synechococcus.
  
  
Line 13: Line 14:
 
===Source===
 
===Source===
  
−
none
+
The sequence of cscB is achieved from the iGEM parts BBa_K3971011. The lac UV5 promoter(BBa_K4115012) is designed by our team. Most components in the composite part are synthesized by the company and then we integrate them into the backbone of PUC57.
  
 
===References===
 
===References===
 +
[1] Lin PC, Zhang F, Pakrasi HB. Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Sci Rep. 2020 Jan 15;10(1):390. doi: 10.1038/s41598-019-57319-5. PMID: 31942010; PMCID: PMC6962321.

Latest revision as of 15:44, 1 October 2022


NS3-2-lac UV5 promoter-cscB-lacI-KanR-NS3-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
    Illegal PstI site found at 4793
    Illegal NgoMIV site found at 1678
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1722


Design Notes

It is proved that cscB can transport sucrose at a good rate with salt stress and IPTG induced.[1] Thus we choose cscB as an exporter in Synechococcus.


Source

The sequence of cscB is achieved from the iGEM parts BBa_K3971011. The lac UV5 promoter(BBa_K4115012) is designed by our team. Most components in the composite part are synthesized by the company and then we integrate them into the backbone of PUC57.

References

[1] Lin PC, Zhang F, Pakrasi HB. Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Sci Rep. 2020 Jan 15;10(1):390. doi: 10.1038/s41598-019-57319-5. PMID: 31942010; PMCID: PMC6962321.