Difference between revisions of "Part:BBa K4324100:Design"

Revision as of 14:29, 1 October 2022

NAD(P)H-dependent D-xylose reductase from S. stipitis

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 747

Design Notes

The protein sequence was codon-optimised for expression in Escherichia coli K12 through the IDT codon optimisation tool as gBlocks® Gene Fragments, and ensured that EcoRI, XbaI, SpeI, and PstI restriction sites were not accidentally present within the CDS.

Source

This original protein sequence was taken from the XYL1 gene of Scheffersomyces stipitis (formerly known as Pichia Stipitis) (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545), which can be found on the Uniprot database.

Alternatively, the protein sequence is pasted below:

MPSIKLNSGYDMPAVGFGCWKVDVDTCSEQIYRAIKTGYRLFDGAEDYANEKLVGAGVKK AIDEGIVKREDLFLTSKLWNNYHHPDNVEKALNRTLSDLQVDYVDLFLIHFPVTFKFVPL EEKYPPGFYCGKGDNFDYEDVPILETWKALEKLVKAGKIRSIGVSNFPGALLLDLLRGAT IKPSVLQVEHHPYLQQPRLIEFAQSRGIAVTAYSSFGPQSFVELNQGRALNTSPLFENET IKAIAAKHGKSPAQVLLRWSSQRGIAIIPKSNTVPRLLENKDVNSFDLDEQDFADIAKLD INLRFNDPWDWDKIPIFV

References

1. https://www.uniprot.org/uniprotkb/P31867/entry 2. https://www.genome.jp/dbget-bin/www_bget?ec:1.1.1.307

@@ Line 7: / Line 7: @@
 ===Design Notes===
-The protein sequence was codon-optimised for expression in Escherichia coli K12 through the [https://sg.idtdna.com/CodonOpt IDT codon optimisation tool] as gBlocks® Gene Fragments, and ensured that EcoRI, XbaI, SpeI, and PstI restriction sites were not accidentally present within the CDS.
+The protein sequence was codon-optimised for expression in '''Escherichia coli K12''' through the [https://sg.idtdna.com/CodonOpt IDT codon optimisation tool] as gBlocks® Gene Fragments, and ensured that EcoRI, XbaI, SpeI, and PstI restriction sites were not accidentally present within the CDS.
 ===Source===