Difference between revisions of "Part:BBa K4408007"

 
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<partinfo>BBa_K4408007 short</partinfo>
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This part is the coding region of the fusion protein of Candida antarctica: lipase B (CALB) and chitin-binding domain (ChBD). It consists CALB (BBa_K2302006), Linker 2 (BBa_K4408004) and ChBD BBa_T2028) sequences. Candida antarctica lipase B (CALB) is a widely used lipase with high stereoselectivity and stability. ChBD is an affinity tag for chitin purification of proteins. ChBD-fusion CALB can facilitate the purification of CALB, thus enhancing the reaction rate of CALB catalyzed esterification.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4408007 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4408007 parameters</partinfo>
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Revision as of 11:50, 1 October 2022


Lipase CALB connected with chitin binding domain

This part is the coding region of the fusion protein of Candida antarctica: lipase B (CALB) and chitin-binding domain (ChBD). It consists CALB (BBa_K2302006), Linker 2 (BBa_K4408004) and ChBD BBa_T2028) sequences. Candida antarctica lipase B (CALB) is a widely used lipase with high stereoselectivity and stability. ChBD is an affinity tag for chitin purification of proteins. ChBD-fusion CALB can facilitate the purification of CALB, thus enhancing the reaction rate of CALB catalyzed esterification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 949
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 670
    Illegal NgoMIV site found at 780
    Illegal AgeI site found at 472
    Illegal AgeI site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1025
    Illegal SapI site found at 964
    Illegal SapI site found at 994