Difference between revisions of "Part:BBa K200009"
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<partinfo>BBa_K200009 short</partinfo>
 
<partinfo>BBa_K200009 short</partinfo>
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This sequence codes for the restriction enzyme DpnII.<br>
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This is a Type II restriction enzyme that recognises the sequence GATC.  Its activity can be blocked by dam methylation.
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The working temperature is 37°C.
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As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.
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===Usage and Biology===
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The restriction enzyme TaqI, is isolated from the bacterium Thermus aquaticus. <cite>1</cite>
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DpnII is a dam sensitive restriction enzyme.<br>
 
DpnII is a dam sensitive restriction enzyme.<br>
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The recognition sequence of this restriction enzyme is GATC and is a 4 base cutter, leaving a 4 base overhang.
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It is a 4 base cutter, leaving a 4 base overhang.
  
 
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<br>
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NCTAGN  ->  NCTAG / N   
 
NCTAGN  ->  NCTAG / N   
  
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As part of the Imperial 2009 iGEM E.ncapsulator project, DpnII was used with TaqI to cut up the genetic material within the cell, completing the cell death module.
 
 
    
 
    
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K200009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K200009 SequenceAndFeatures</partinfo>

Revision as of 17:23, 2 September 2009

DpnII Composite

This sequence codes for the restriction enzyme DpnII.

This is a Type II restriction enzyme that recognises the sequence GATC. Its activity can be blocked by dam methylation.

The working temperature is 37°C.

As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.


Usage and Biology

The restriction enzyme TaqI, is isolated from the bacterium Thermus aquaticus. 1


DpnII is a dam sensitive restriction enzyme.

It is a 4 base cutter, leaving a 4 base overhang.


The cutting site is as shown:
NGATCN -> N / GATCN
NCTAGN -> NCTAG / N


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[http://www.neb.com/nebecomm/products/productR0543.asp Information from NEB]