Difference between revisions of "Part:BBa K200008"
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Furthermore, the presence of oxygen and an additional cofactor (TPNH) is required for the hydrolysis to take place <cite>#PAH4</cite>. | Furthermore, the presence of oxygen and an additional cofactor (TPNH) is required for the hydrolysis to take place <cite>#PAH4</cite>. | ||
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Revision as of 17:01, 2 September 2009
Phenylalanine hydroxylase
[http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase Phenylalanine hydroxylase (PAH)] is the enzyme that breaks down [http://en.wikipedia.org/wiki/Phenylalanine phenylalanine] to [http://en.wikipedia.org/wiki/Tyrosine tyrosine]. Deficiency of this enzyme activity results in the autosomal recessive disorder [http://en.wikipedia.org/wiki/Phenylketonuria phenylketonuria].#PAH1
Usage and Biology
PAH works well at a PH of around 7.0 (similar to the small intestine), and is denatured at temperatures above 30 degrees celsius (in experiments with gram-negative bacteria)#PAH2. The enzyme can be rendered protease resistant via full phosphorylation.#PAH3
Furthermore, the presence of oxygen and an additional cofactor (TPNH) is required for the hydrolysis to take place #PAH4.
Requirements
- Only works in gram negative bacteria
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 287
Illegal BamHI site found at 814
Illegal XhoI site found at 524 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta Sequence from NCBI]
<biblio>
- PAH1 pmid=2014036
- PAH2 pmid=15708798
- PAH3 pmid=8573072
- PAH4 pmid=13525410
</biblio>