Difference between revisions of "Part:BBa K4220008"
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==References== | ==References== | ||
Latest revision as of 07:11, 1 October 2022
P2Y2-P2A-mCherry
Introduction
The product coded by this sequence is used to show the position of P2Y2 and the function of P2A by mCherry reporter.
- P2Y2
- In order to increase its sensitivity and maximum response to sense eATP (extracellular ATP), we mutated K240N and F307S.[1]
- P2A
- After adding two proteins in the two sides of P2A, it will cleave by itself and release the two proteins at the same time.
- mCherry
- To report the successful cleavage of P2A peptide.
Results
1.The P2A peptide can work effectively:
2.The production of P2Y2:
References
1 Scott, B. M. et al. Self-tunable engineered yeast probiotics for the treatment of inflammatory bowel disease. Nature Medicine 27, 1212-1222, doi:10.1038/s41591-021-01390-x (2021).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1159
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1095
Illegal AgeI site found at 389 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 570
Illegal SapI site found at 615